Radiochemical purities were always greater than 97% without high-performance liquid chromatography purification. These synthetic approaches hold considerable promise as rapid and simple method for the radiofluorination of folate derivatives with high radiochemical yield in short synthesis time. In vitro tests on KB cell line showed that significant amount of the radioconjugates were associated with cell fractions, and in vivo characterization in normal Balb/c mice revealed rapid blood clearance of these radioconjugates with excretion predominantly by the urinary and partially by the hepatobiliary
systems. Biodistribution studies click here in nude mice bearing human KB cell line xenografts demonstrated significant tumor uptake and favorable biodistribution profile for [(18)F]-2-folate over the other conjugates. The uptake in the tumors was blocked by excess coinjection of folic acid, suggesting a receptor-mediated process. Micro-positron emission tomography images
of nude mice bearing human KB cell line xenografts confirmed these observations. These results demonstrate that [(18)F]-2-folate may be useful as molecular probe for detecting and staging of folate receptor-positive cancers, such as ovarian cancer and their metastasis as well as monitoring tumor response to treatment. (C) 2011 Elsevier Inc. All rights reserved.”
“Background Human papillomaviruses (HPVs) cause genital warts and cancers in men. The natural history of HPV infection in men is largely unknown, and that information is needed to inform prevention strategies. The goal in this study was to estimate incidence and clearance of type-specific Rigosertib ic50 genital HPV infection in men, and to assess the associated factors.
Methods Men (aged 18-70 years), residing in Brazil, Mexico, and the USA, who were HIV negative and reported no history of cancer were recruited from the general population, universities, and organised health-care systems. They were assessed every 6 months for a median follow-up of 27.5 months (18.0-31.2). selleckchem Specimens from the coronal sulcus, glans penis, shaft, and scrotum were obtained for the assessment of the status of HPV genotypes.
Findings
In 1159 men, the incidence of a new genital HPV infection was 38.4 per 1000 person months (95% CI 34.3-43-0). Oncogenic HPV infection was significantly associated with having a high number of lifetime female sexual partners (hazard ratio 2-40, 1-38-4-18, for at least 50 partners vs not more than one partner), and number of male anal-sexual partners (2.57, 1.46-4.49, for at least three male partners vs no recent partners). Median duration of HPV infection was 7-52 months (6.80-8.61) for any HPV and 12.19 months (7.16-18.17) for HPV 16. Clearance of oncogenic HPV infection decreased in men with a high number of lifetime female partners (0.49, 0.31-0.76, for at least 50 female partners vs not more than one partner), and in men in Brazil (0.71, 0.56-0.