Transcription factor evaluation uncovered that 462 transcription factors have been noticed inside the genome of P. aurantiogriseum NRRL 62431 which includes C2H2, C6, Zn 2Cys6, GATA, HACA, APSES, HLH, bZIP, STP8, NF Y, SRE, CP2, PHD, RFX, Examination of membrane transporters during the genome of P. aurantiogriseum NRRL 62431 recognized a complete of 113 predicted multidrug transporters that are presumably in volved in transportation and detoxification of secondary metabolites, Among them, 93 belong to ABC transporters, In order to determine genes concerned in paclitaxel biosyn thesis in P. aurantiogriseum, a protein search was carried out towards the genome of P. aurantiogriseum NRRL 62431 implementing the 13 reported paclitaxel biosyn thetic genes in Taxus.
This search exposed putative ho mologs to 7 genes encoding phenylalanine aminomutase, geranylgeranyl diphosphate synthase, taxane five hydroxylase, taxane 13 hydroxylase, taxane 7B hydroxylase, taxane 2 hydroxylase and taxane 10B hydroxylase of Taxus, Furthermore, an acyltransferase was identified from the P. aurantiogriseum NRRL 62431 gene set by BLASTp Aurora B inhibitor search towards GenBank databases. Comparative examination of paclitaxel biosynthetic genes involving P. aurantiogriseum NRRL 62431 and its host Potential paclitaxel biosynthetic gene homologs with identity 30% for the 13 reported paclitaxel biosynthetic genes were found while in the paclitaxel generating hazel, The most conserved genes had been GGPS and PAM with amino acid identities of 62% and 63%, re spectively, Comparison of your paclitaxel biosynthetic gene candidates in host hazel against P.
aurantiogriseum NRRL 62431 genome showed that their paclitaxel bio synthetic genes have been not remarkably conserved, sharing only 21% to 62% sequence identities, One other strain of endophytic fungus P. aurantiogri seum was also isolated in the host plant Taxus baccata and was shown to synthesize taxane, We in contrast P. aurantiogriseum NRRL selelck kinase inhibitor 62431 genome against the paclitaxel genes in T. baccata and once more uncovered paclitaxel biosynthetic gene candidates in P. aurantiogriseum NRRL 62431 and paclitaxel biosynthetic genes in T. baccata were rather various, only 19% to 65% identical in amino acid sequences, Comparative evaluation of P. aurantiogriseum NRRL 62431 with an endophytic fungus EF0021 Recently the genome of an endophytic fungus EF0021 isolated from Taxus spp. that was incapable of independ ent paclitaxel synthesis was sequenced, Comparison with the paclitaxel biosynthetic candidate genes from P. aurantiogriseum NRRL 62431 with EF0021 unveiled only likely similarity to PAM, GGPPS, and p450, Phylogenetic analysis of P. aurantiogriseum NRRL 62431 Comparison of your P. aurantiogriseum NRRL 62431 and F.