Table 2 Effect of growth CFTRinh-172 mouse condition on intra- and extra-cellular iron concentrations and gene regulation Parameter tested Growth condition   Aerobic Microaerobic   LVS Δ mglA FUU301 LVS Δ mglA FUU301 Fe intraa 626 ± 27.2 661 ± 17.1 643 ± 24.5 893 ± 33.8 589 ± 21.9d 662 ± 20.5d Fe extrab B.D.L.e 186 ± 20.5 64.5 ± 8.97 73.9 ± 19.3 327 ± 10.7d 165 ± 46.1 Gene regulationc fslA 12.7 ± 0.64 2.51 ± 0.19f 10.6 ± 1.33 5.87 ± 0.71 4.93 ± 0.48 9.29 ± 1.19g fslB 6.27 ± 0.39 0.83 ± 0.15f 5.6 ± 1.09 2.86 ± 0.43 1.87 ± 0.30 5.86 ± 0.30 fslC

5.96 learn more ± 0.36 0.74 ± 0.15f 4.86 ± 0.68 2.61 ± 0.33 1.55 ± 0.28g 4.69 ± 0.26g fslD 3.19 ± 0.23 0.97 ± 0.15f 3.52 ± 0.35 1.60 ± 0.23 2.40 ± 0.27g 3.73 ± 0.37g fslE 0.82 ± 0.24 1.11 ± 0.15 1.55 ± 0.20h 1.04 ± 0.06 1.98 ± 0.14d 5.43 ± 1.20d feoB 4.03 ± 0.29 1.37 ± 0.15f 4.95 ± 0.27 5.50 ± 0.41 4.33 ± 0.52 12.8 ± 3.77 katG 50.7 ± 8.62 110 ± 15.3h 116 ± 18.21h 79.1 ± 7.14 120 ± 19.3 135 ± 12.2i iglC 390 ± 140 24.6 ± 5.37f 385 ± 58 685 ± 159 38.5 ± 15.9d 478 ± 120 mglA 16.5 ± 5.77 B.D.L. 637 ± 173g a The intracellular iron pool (ng/OD600 nm) of the strains after 18 h of growth b Iron (ng/ml) remaining in the culture medium after 18 h of growth c The expression of the genes was Fossariinae analyzed by quantitative real-time PCR. Results are expressed as RCN means ± SEM of results from four

independent samples d P < 0.001 https://www.selleckchem.com/products/btsa1.html relative to LVS in the microaerobic condition e Below Detection Limit f P < 0.001 relative to LVS in the aerobic condition g P < 0.05 relative to LVS in the microaerobic condition h P < 0.05 relative to LVS in the aerobic condition i P < 0.01 relative to LVS in the microaerobic condition Compared to the aerobic conditions, LVS down-regulated fslA-D 2.5-fold under microaerobic conditions, whereas, in contrast, ΔmglA expressed 2-fold more of fslA-D microaerobically than aerobically. Overall, the adaptations under microaerobic conditions meant that fslA-C and feoB were expressed slightly higher and fslD and fslE almost 2-fold lower in LVS than ΔmglA (Table 2). The fsl genes were expressed at similar levels, and feoB was upregulated about 3-fold in FUU301 when cultivated in the microaerobic versus the aerobic milieu. In summary, we observed that ΔmglA very markedly down-regulated the fslA-D and feoB genes compared to LVS under aerobic conditions but that differences were only marginal microaerobically, despite that less iron was present when ΔmglA had been cultivated under aerobic conditions. This supports our hypothesis that ΔmglA is subjected to oxidative stress under aerobic conditions and therefore needs to minimize iron uptake as a compensatory mechanism to avoid toxic effects of the Fenton reaction.