The results of growth curve assay and colony formation assay showed that the growth and proliferation of the cell strains with stable expression of FBG2 were significantly faster than those of the cells transfected with empty vectors and OSI 906 untreated control cells not only in gastric cancer cell line but also in normal gastric cell line. Therefore, FBG2 gene could accelerate the growth and proliferation of cells. The reasons might be as follows: (1) The gene products promoted the activities of the metabolic system of ubiquitin so as to enhance the metabolism of some protein molecules in cells and accelerate the growth and proliferation of

cells. (2) It might accelerate the degradation of proteins inhibiting the growth and proliferation of eFT508 solubility dmso cells so as to promote the growth and proliferation. The results of flow cytometry assay showed that the proportions of

cells in G2-M phase in the cell strains with stable expression of FBG2 were higher than those of the control groups and the proportions of cells in S phase were lower than those of the control cells. Corinna Benz[17] thought that F-box proteins could control cell cycle by adjusting the degradation of some proteins which controlled cell cycle such as cyclins. The division cycle of eukaryotic cells is controlled by protein kinases which are activated by binding to cyclins. Cyclins are present only at particular times in the cell cycle; after they are no longer required they are destroyed by ubiquitination followed by digestion by the proteasome [18–20]. The ubiquitin chains are added by Depsipeptide ic50 a cascade of enzymes called E1, E2 and E3 ubiquitin ligases, and specificity is determined by the E3 components. The E3 ligases that are

important for cell cycle control are the anaphase promoting complex or cyclosome (APC/C) and the Skp1-Cdc53/cullin-F-box protein (SCF) complex [21]. In SCF complexes, proteins with an “”F-box”" domain (also called “”cyclin-like F-box”") link check details targets to the degradation machinery. There was no significant difference of the apoptosis rates between each group. The results indicated that for the cell strains with stable expression of FBG2, many were in the division stage, so FBG2 gene could accelerate the growth and proliferation of cells. However, this gene did not affect the apoptosis of gastric cancer cells or normal gastric cells perhaps because FBG2 gene or the metabolic system of ubiquitin had little influence on the key genes concerned with apoptosis procedure. The results of Transwell migration assay showed that there was no significant difference in the migration capacity, which represented the invasiveness of cells, between each groups of these cells and the cause needed to be further investigated.