RyhB); f) highest Mascot score for a protein from LC-MS/MS or MALDI data; g) Vs (-Fe): average spot volume (n ≥ 3) in 2D gels for iron-depleted

growth conditions at 26°C as shown in Figure 3; h) Vs (+Fe): average spot volume (n ≥ 3) in 2D gels for iron-supplemented growth conditions at 26°C; i) spot volume ratio (-Fe/+Fe) at 26°C, N.D.: not determined; -: no spot detected; j) two-tailed t-test p-value for spot abundance change Torin 1 solubility dmso at 26°C, 0.000 see more stands for < 0.001; k) average spot volume ratio (-Fe/+Fe) at 37°C; additional data for the statistical spot analysis at 37°C are part of Additional Table 1. Experimental pI values span a pH range because proteins were visualized as spot trains. Table 3 Abundance differences of Y. pestis proteins profiled in cytoplasmic fractions of iron-rich vs. iron-starved cells Spot No a) Gene locus b) gene name c) Protein description c) Subc. d) Fur/RyhB e) Mascot Score f) exp Mr (Da) exp pI 26°C, Vs (-Fe) g) 26°C, Vs (+Fe) h) 26-ratio -Fe/+Fe i) 26°C P-value ACP-196 j) 37-ratio -Fe/+Fe k) 1 y0015 aceB malate synthase A CY Fur 688 63974 5.86 0.06 1.73 0.036 0.000 0.421 2 y0016 aceA isocitrate lyase CY   741 54571

5.47 0.38 4.19 0.090 0.000 0.408 3 y0047 glpK glycerol kinase CY   828 60235 6.01 0.07 0.33 0.198 0.000 0.570 4 y0320 oxyR DNA-binding transcriptional regulator OxyR CY   510 36649 5.82 0.49 0.40 1.237 0.004 0.791 5 y0548 metF2 putative methylenetetrahydrofolate reductase U   321 31848 5.73 1.77 1.06 1.677 0.000 0.543 6 y0617 frdA fumarate reductase, anaerobic, flavoprotein subunit PP   437 80764 5.77 – 0.23 < 0.05 N.D. < 0.05 11 y0854 fumA fumarase A CY RyhB 255 68184 6.02 - 0.50 < 0.05 N.D. < 0.05 12 y0870 katY catalase; hydroperoxidase HPI(I) U   768 78569 6.32

0.11 0.48 0.231 0.000 0.081 13 y0888 luxS predicted S-ribosylhomocysteinase CY   670 19733 5.46 0.79 0.30 2.617 0.000 2.164 14 y0988 ahpC putative peroxidase selleck compound CY   898 24298 5.75 5.02 6.10 0.823 0.202 1.376 15 y1069 ymt murine toxin U   7052 67771 5.64 13.61 9.61 1.415 0.143 1.359 16 y1069 ymt murine toxin, C-t. fragment U   245 33893 5.30 0.89 0.19 4.634 0.000 N.D. 17 y1069 ymt murine toxin, N-t. fragment U   164 39074 6.11 0.84 0.17 4.860 0.000 N.D. 18 y1208 fur ferric uptake regulator CY   95 13425 6.16 0.11 0.17 0.651 0.055 N.D. 19 y1282 yfiD formate acetyltransferase, glycyl radical cofactor GrcA CY   521 13866 4.75 1.27 2.27 0.560 0.000 0.456 20 y1334 iscS selenocysteine lyase/cysteine desulfurase U   408 51519 5.96 – - N.D. N.D. 0.59 21 y1339 hscA chaperone protein HscA CY   384 49149 5.53 – - N.D. N.D. 0.