Results are expressed as seconds (s) or ratio Determination of C

Results are expressed as seconds (s) or ratio. Determination of C-reactive protein level We quantitatively determined CRP levels using an immune turbidimetric assay. Latex particles coated with antibody specific to human CRP aggregated in the presence of CRP from the sample, and formed immune complexes. These immune complexes resulted in an increase in light scattering proportional to the CRP concentration MG132 protocol in the serum sample. Light scattering was measured using turbidity (absorbance) readings. CRP concentration was determined using a calibration curve developed from CRP standards of known concentrations (multistandard), with a linearity of the method range of 0.2�C480 mg/dL.

Determination of D-dimer level D-D levels were determined using a quantitative enzyme-linked immunosorbent assay based on the ��sandwich�� type immunoenzymatic method, with final measurements achieved through fluorescence detection. Assays were performed using a VIDAS Automated Immunoassay System (Biomerieux), with a measurement range of 45�C10.000 ��g/L. The reference value used was <500 ��g/L. Determination of platelet count PLT count was automatically measured using a Pentra 80 cell-counter (Horiba), and confirmed by an indirect manual counting method involving a blood smear that was collected from the patient��s finger. After collection, the blood smear was fixed in methanol for 10 min, and then stained with Giemsa for 20 min before being used. Statistical analysis Continuous data are presented as average value and standard deviation. The student��s t-test for paired samples used to compare values between the 2 stages.

Graphic lines were used for data presentation. The relationship between 2 variables was analyzed using Pearson��s and Kendall��s correlation coefficients. A difference with a p value less than 0.05 (p < 0.05) was considered statistically significant. Data analysis was carried out using the Statistical Package for the Social Sciences 11.5 software (SPSS, Chicago, IL). Results Prothrombin time Data analysis using student��s t-test for paired samples showed a statistically significant difference between PT values before surgery (PT 1) and after surgery (PT 2; t = 3.446, df = 44, p = 0.001; Fig. 1). Fig. 1 PT values before surgery (PT 1) and after surgery (PT 2). The mean PT value declined from 90.38% before surgery to 81.25% after surgery.

Activated partial thromboplastin time Data analysis using student��s t-test for paired samples showed no statistically significant difference between preoperative and postoperative APTT (t = 1,406, df = 44, p = 0.167; Fig. 2). Fig. 2 Pre- and postoperative APTT values. In our study, Entinostat we observed no statistically significant changes in APTT values before and after surgery (33.96 s versus 35.07 s, respectively). This observation could be explained by the fact that patients were receiving heparin medication in prophylactic but not therapeutic doses.

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