A library of PAC 1 analogues was created with the goal of identifying materials able to eliciting strong death of cancer cells in culture. Described synthetic routes to PAC 1 and S PAC 1, together with other PAC 1 analogues, as the supplier Doxorubicin final step up the synthetic structure employ the condensation of a hydrazide and an aldehyde. 18 As demonstrated in Figure 2, 31 hydrazides and 27 aldehydes were chosen for making the library of 837 PAC 1 analogues. The hydrazides were made out of commercially available benzyl halide starting materials. The syntheses of hydrazides 1 have now been described previously. 8, 18, 23 Hydrazides 1 were synthesized in accordance with Scheme 1. Substituted benzyl halides 4 first responded with piperazine to make substituted benzylpiperazines 5. A second alkylation of the piperazine ring with ethyl chloroacetate gave disubstituted 6 to piperazines, and the esters Organism were then transformed into hydrazides 1 by reaction with hydrazine. The artificial routes toward hydrazides 1 are step-by-step in Scheme 2. Synthesis of hydrazide 1 started from the alkylation of piperazine with 4 vinylbenzyl chloride to make monosubstituted piperazine 8. A second alkylation with ethyl chloroacetate formed ester 9, and reaction with hydrazine giving hydrazide 1 and paid down the olefin, formed the hydrazide. The reduction of olefins with hydrazine generally involves the addition of an oxidizing agent,25 but the presence of atmospheric oxygen was sufficient to make this happen change. Activity of hydrazide 1 began with the result of ethyl 2 acetate, produced as formerly reported,8 with benzyl bromide 4 to form intermediate 6. Reaction of 6 with hydrazine then established hydrazide 1. Hydrazide 1 was synthesized MAPK phosphorylation start with the reaction of 1 phenylpiperazine with ethyl chloroacetate to provide disubstituted piperazine 6, and reaction with hydrazine established hydrazide 1. Hydrazide 1, was produced by first protecting as the ethylene acetal 4 methylbenzophenone, as shown in Scheme 2, equation 4. This compound was brominated under radical conditions to offer benzyl bromide 13. Reaction with monosubstituted piperazine 10 gave advanced 14, and response with hydrazine gave hydrazide 15. Employing a Bchi Syncore parallel synthesizer, each hydrazide was condensed with each aldehyde, with more than 80 reactions done simultaneously.