, 2009b). The sampling site, Puerto Cuatreros station (38°50′ S; 62°20′ W), is a shallow harbor (mean depth: 7 m) located at the head of the estuary (Fig. 1) and characterized by a restricted circulation (tidal velocities between 0.69 and 0.77 m s−1), low advection and a relatively long residence time (ca. 30 days).

The Obeticholic Acid cost river runoff is low; the Sauce Chico River, the main freshwater tributary, presents a mean annual runoff of 1.9 m3 s−1, with maximum of 106 m3 s−1 in autumn due to rainfalls, and the Napostá Grande Creek has an annual runoff of 0.8 m3 s−1 (Melo and Limbozzi, 2008). The maximal plankton biomass of the estuary is found in the inner zone of the estuary (Barria de Cao et al., 2005, Berasategui et al., 2013 and Popovich and Marcovecchio, 2008) which is highly eutrophic due to important inputs of organic matter, detritus and nutrients from anthropogenic sources (industrial, urban and agricultural activities) (Freije et al., 2008) and saltmarshes (Montemayor et al., 2011 and Negrin et al., 2013). In this area, numerous interconnected channels separate small islands and vast tidal flats and saltmarshes with halophytes of the species Sarcocornia perennis, Spartina alterniflora and S. densiflora ( Isacch et al., 2006). The extensive bare flats are mainly composed of silt-clay sediments covered with Everolimus research buy dense microbial mats ( Cuadrado and Pizani, 2007 and Parodi and Barría

de Cao, 2003). Benthic fauna is dominated by Laeonereis acuta, a deposit-feeder polichaete, and the burrowing crab Neohelice granulata ( Escapa et al., 2007). The sampling was carried out on a fortnightly frequency from January to December 2007 at Puerto Cuatreros station, during midday and high tides. Mean depth of the sampling station was 7 m. Surface water temperature was measured in situ using a portable Horiba U-10 multi-probe (Horiba Ltd., Kyoto, Japan). Water samples were collected from the surface (approx. 0.5 m depth), using a van Dorn bottle (2.5 l), stored in a cooler and taken to the laboratory to estimate phytoplankton

abundance, chlorophyll a (chl), phaeopigments (pha) and dissolved inorganic nutrient concentrations (nitrate, nitrite, phosphate and silicate) and particle size concentration. Samples Levetiracetam for phytoplankton enumeration were preserved with acid Lugol’s solution. For the taxonomic identification of the species, water samples were collected with a Nansen net (30 μm mesh) and preserved with formalin (final concentration 4%, v/v). For the purpose of this work, here we only present the phytoplankton species succession from May to November (winter-mid spring), which corresponds to the bloom and post-bloom periods ( Guinder et al., 2009b and Popovich et al., 2008). In addition, mesozooplankton samples were collected from July to October 2007, with a plankton net (200-μm mesh) by means of subsurface horizontal tows (0.5 m depth) and were preserved in 4% buffered formalin.