121 Thus, activation of myeloid APCs via exposure to certain

types of TLR ligands may result in the biosynthesis of different self lipids that are not yet identified but that may be stronger agonists for iNKT cells than the lipids presented by non-activated APCs (Fig. 3a). Our recent discovery that a substantial fraction of human iNKT cells recognize lyso-phosphatidylcholine (LPC) as a self antigen suggests a mechanism by which antigen abundance may be connected to endogenous signalling pathways.122 One of the first things to happen Apoptosis Compound Library cell line upon stimulation of myeloid cells by growth factors, cytokines, neurotransmitters, hormones, and danger signals such as TLR ligands is the activation of phospholipase A2 (PLA2) enzymes.123,124 PLA2 cleaves SB431542 nmr the sn-2 acyl chain bond of phosphatidylcholine (PC), one of the most abundant membrane lipids in eukaryotic cells, releasing LPC and a free fatty acid (Fig. 3b). The free fatty acids produced by this process are the biochemical substrates

for the synthesis of lipid mediators such as leukotrienes, prostaglandins and lipoxins which are critical elements in the regulation of inflammation.125,126 LPC can itself serve as an intercellular lipid messenger or it may be further chemically modified, for example by an acetylation reaction that produces platelet-activating factor.125,127 Thus, the finding that many iNKT cells recognize LPC as a CD1d-presented antigen provides a novel molecular link between these innate regulatory T cells and the initiation point of the biosynthesis

of lipid mediators that have key roles in inflammation. As LPC is generated during the course of normal cellular growth processes, it is probably constitutively presented by CD1d molecules on APCs. Indeed, recent analyses have identified LPC as one of the types of cellular lipids bound to human CD1d molecules.128,129 However, it is also known that during acute and chronic inflammatory states the levels of both LPC and secreted PLA2 enzymes can rise dramatically www.selleck.co.jp/products/Verteporfin(Visudyne).html in serum and other extracellular fluids, and therefore it is reasonable to suppose that the amount of LPC presented by CD1d might increase under inflamed conditions, and that this might cause enhanced iNKT cell activation (Fig. 3b). A further possibility suggested by our data, however, is that at some point the LPC concentrations may become inhibitory and may fail to induce iNKT cell activation, suggesting that this pathway may shut down under conditions of very strong or prolonged inflammation.122 It is also interesting to note that another report has described the expansion of LPC-reactive CD1d-restricted T cells that are not iNKT cells (i.e. a population of type II NKT cells) in blood of human multiple myeloma patients.