Bcl 2 exerts an anti apoptotic effect by inhibiting mitochondrial outer membrane permeabilization to suppress release of cytochrome c to the cytosol. Bcl 2 could also inhibit necroticlike cell death by blocking opening of the mitochondrial permeability transition pore to keep up cellular ATP levels within survival ubiquitin conjugating limitations. Cell death can be blocked by forced overexpression of Bcl 2 created by a variety of stimuli, including cyanide. In this study it was observed that over expression of Bcl 2 blocked development of cyanide poisoning by UCP 2 up legislation. It appears that the cell death is born partly to paid down Bcl 2 levels and transfection with Bcl 2 cDNA increased Bcl 2 term which then permitted the cells to keep survival. Bcl 2 expression is controlled at both transcriptional and post transcriptional levels. Whereas post translational modifications, including Immune system dephosphorylation and ubiquitination, are crucial for purpose and stability of the protein under various pathologic conditions, transcriptional regulation controls expression, as shown by mRNA levels. In this study, cyanide considerably reduced Bcl 2 levels in UCP 2 up regulated cells. Since degrees of Bcl 2 mRNA weren’t altered in comparison with constitutive expression, it appeared that article transcriptional events were involved in the down-regulation. Proteasome inhibition blocked Bcl 2 down regulation, therefore improved proteasomal degradation likely mediated the reduction in protein levels. Bcl 2 degradation is stimulated by oxidative stress, including mitochondrial generation of HOPeroxides market Bcl 2 proteasomal metabolism by causing dephosphorylation and ubiquitination. In cells undergoing UCP 2 up legislation, cyanide increased HOgeneration. The increased oxidative stress then mediated Bcl 2 destruction since pretreatment with catalase, a HOscavenger, blocked the down regulation deubiquitination assay of Bcl 2. In mitochondria, GSH is a must for keeping redox homeostasis and protection against HOmtGSH depletion results in HOaccumulation to boost cellular oxidative damage. Diminished mtGSH levels have been related to a reduced amount of Bcl 2 expression and increased apoptosis. UCP 2 up legislation improved cyanide mediated destruction of mtGSH, ergo improving cellular accumulation of HOand subsequently stimulating Bcl 2 degradation. Pretreatment with GSH EE restored mtGSH levels and blocked Bcl 2 down regulation, hence indirectly showing mtGSH exhaustion led towards the reduction and oxidative stress of Bcl 2 term. The decrease of cellular GSH following exposure to cyanide is probable due simply to paid down cellular ATP resulting from inhibition of cytochrome c oxidase. Furthermore, inhibition of mitochondrial oxidative phosphorylation influences ROS production, leading to paid down mtGSH. In this review, UCP 2 up regulation improved cyanide exhaustion of mtGSH.