hollisae strains. Distribution of tdh or trh in T3SS2-positive Seliciclib manufacturer V. cholerae and V. mimicus strains In V. parahaemolyticus, the strains which possess the T3SS2 gene cluster also possess the tdh and/or trh genes [7, 20]. To examine whether the tdh or trh genes also coexist in T3SS2-possessing V. cholerae and V. mimicus strains, we performed a PCR assay using the pair of primers (see Additional
file 1) which target the tdh or trh genes of V. parahaemolyticus RIMD2210633 or TH3996 strains, respectively. Among the T3SS2-possessing V. mimicus strains, the tdh gene could be detected in all T3SS2α-positive strains, although no Vadimezan ic50 amplicons of the trh gene could be obtained in V. mimicus strains, while neither of the amplicons could be obtained in the T3SS2-positive V. cholerae strains. To analyze the distribution of the tdh or trh genes in 12 T3SS2-positive V. cholerae strains, we performed an additional PCR assay using the primer set (see Additional file 1) targeting the region between A33_1702 and the downstream region of the V. cholerae AM-19226 strain which is homologous with tdh of V. parahaemolyticus. PCR products could be obtained for both the T3SS2α- and T3SS2β-positive V. cholerae strains, except for the RIMD2214321 (T3SS2α-possessing) strain (data not shown).
These results suggest that the tdh gene may be related to the presence of the T3SS2 gene cluster in V. cholerae and V. mimicus strains. Gene organization Niclosamide of the T3SS2 gene cluster in V. mimicus The results presented here demonstrated that some V. mimicus Nutlin-3a supplier strains possess the genes for T3SS2α or T3SS2β. Since the gene organizations of the
T3SS2 gene cluster in the organism were completely unknown, we attempted to analyze the gene organization of the T3SS2 region in V. mimicus strains. To this end, we performed PCR scanning against the genomic DNA of T3SS2-positive V. mimicus strains RIMD2218080 (T3SS2α) and RIMD2218067 (T3SS2β) by using six PCR primer pairs for each strain (see Additional file 3). In the T3SS2α-positive strain RIMD2218080, PCR products of the expected size were detected for all primer pairs (data not shown), thus suggesting that the gene organization of the T3SS2 gene cluster in strain RIMD2218080 is similar to that of the T3SS2α gene cluster in V. parahaemolyticus strain RIMD2210633. In the T3SS2β-positive strain RIMD2218067, amplicons of the expected size were obtained for five primer pairs, although the size of the product obtained for a primer pair between the vopD2 and vopC genes was notably larger, by approximately 5 kb, than that of the region of the V. parahaemolyticus TH3996 (data not shown). However, the PCR product which was amplified between vopD2 and vopP was the same size as that of the V. parahaemolyticus TH3996 strain. This suggested that the gene organization of T3SS2 of V. mimicus RIMD2218067 is similar to that of T3SS2β of V.