The authors thank Dr B. Leete (Zeiss Microscopy UK) for help with image processing and analysis. Drs K.M. Sousa (University of Michigan) and O. Kiehn (Karolinska Institute) are acknowledged for their critical comments on this manuscript. This work was supported by the Scottish Universities Life Science Alliance (T. Harkany), Alzheimer’s Association (T. Harkany), Alzheimer’s Research Trust (ART) UK (J.M. & Doxorubicin manufacturer T. Harkany), European Molecular Biology Organization Young Investigator Programme (T. Harkany), National Institutes of Health grant DA023214 (T. Harkany, Y.L.H.), Swedish Medical

Research Council (T. Hökfelt, T. Harkany), European Commission (HEALTH-F2–2007-201159; T. Harkany), Grants-in-Aid for Selleckchem BTK inhibitor Scientific Research from the MEXT, Japan (Y.Y.), Takeda Science Foundation (Y.Y.), and Knut and Alice Wallenberg Foundation (M.U.). J.M. is the recipient of a postdoctoral fellowship from ART UK. L.S. is a Medical Research Council-Integrated Toxicology Training Partnership (MRC-ITTP) postgraduate fellow. Abbreviations BST bed nucleus

of stria terminalis CA central amygdaloid nucleus CB calbindin D28k CBP Ca2+-binding protein ChAT choline-acetyltransferase CR calretinin Cy carbocyanine DRG dorsal root ganglion E embryonic day EA extended amygdala GAD glutamic acid decarboxylase GE ganglionic eminence GP globus pallidus IPAC interstitial nucleus of the posterior limb of the anterior commissure MA medial amygdaloid nucleus OB olfactory bulb qPCR quantitative (real-time)

PCR P postnatal day PB Na-phosphate buffer PFA paraformaldehyde Cediranib (AZD2171) PV parvalbumin scgn secretagogin SI substantia innominata VP ventral pallidum Fig. S1. Quality control of qPCR reactions. Fig. S2. Comparison of polyclonal antibodies raised against secretagogin. Fig. S3. Comparative anatomy of mid-gestational lemur and mouse embryos. Table S1. Nomenclature of brain regions and their list of abbreviations used in this report. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset by Wiley-Blackwell. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. “
“Loss of dopaminergic neurons in Parkinson’s disease (PD) and PD animal models has been extensively documented to cause global changes in electrophysiological activity throughout the cortico-basal ganglia network. However, such loss is also associated with a range of morphological alterations of neurons forming this network, most notably the medium spiny neurons (MSNs) that are the main output neurons of the striatum.